gel mobility shift

gel mobility shift
(= gel retardation assay)
Technique for studying DNA-protein interactions. For example, to study the levels of a particular transcription factor, nuclear protein extracts are incubated with a radiolabelled DNA fragment containing the transcription factor&’s binding site. When the DNA gel is run, the amount of radiolabel that runs more slowly than the free DNA is directly proportional to the amount of transcription factor present.

Dictionary of molecular biology. 2004.

Игры ⚽ Поможем сделать НИР

Look at other dictionaries:

  • Electrophoretic mobility shift assay — An electrophoretic mobility shift assay (EMSA), also referred as a gel shift assay, gel mobility shift assay, band shift assay, or gel retardation assay, is a common technique used to study protein DNA or protein RNA interactions. This procedure… …   Wikipedia

  • Electrophoretic Mobility Shift Assay — Der Electrophoretic Mobility Shift Assay (EMSA) oder Band Shift Assay dient zum Nachweis von DNA oder RNA bindenden Proteinen, beispielsweise Transkriptionsfaktoren. Weitere Möglichkeiten bestehen darin, Protein Liganden Interaktionen jeglicher… …   Deutsch Wikipedia

  • Electrophoretic Mobility Shift Assay — Retard sur gel Pour les articles homonymes, voir EMSA. Le retard sur gel (EMSA ou electrophoretic mobility shift assay) est une technique de biologie moléculaire permettant de détecter une interaction entre une protéine et de l ADN ou de l ARN.… …   Wikipédia en Français

  • electrophoretic mobility shift assay — gel retardation a …   Medical dictionary

  • Gel electrophoresis — apparatus – An agarose gel is placed in this buffer filled box and electrical field is applied via the power supply to the rear. The negative terminal is at the far end (black wire), so DNA migrates toward the camera. Classification… …   Wikipedia

  • gel retardation assay — (= mobility shift assay) Test for interaction between molecules by looking for a change in gel electrophoretic mobility. For example, to assay for levels of a transcription factor, cell extracts are incubated with a radiolabelled oligonucleotide… …   Dictionary of molecular biology

  • Retard sur gel — Pour les articles homonymes, voir EMSA. Le retard sur gel (EMSA ou electrophoretic mobility shift assay) est une technique de biologie moléculaire permettant de détecter une interaction entre une protéine et de l ADN ou de l ARN. Pour cela, on… …   Wikipédia en Français

  • Zinc finger chimera — Zinc finger protein chimera are chimeric proteins composed of a DNA binding zinc finger protein domain and another domain through which the protein exerts its effect. The effector domain may be a transcriptional activator (A) or repressor… …   Wikipedia

  • List of molecular biology topics — This is a list of topics in molecular biology. See also list of biochemistry topics. NOTOC # 3 end 3 flanking region 5 end 5 flanking region 5 ribose 3 A acrylamide gels adenine adenosine deaminase deficiency adenovirus agarose gel… …   Wikipedia

  • Northern blot — The northern blot is a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a sample.[1][2] …   Wikipedia

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”