gel mobility shift

gel mobility shift
(= gel retardation assay)
Technique for studying DNA-protein interactions. For example, to study the levels of a particular transcription factor, nuclear protein extracts are incubated with a radiolabelled DNA fragment containing the transcription factor&’s binding site. When the DNA gel is run, the amount of radiolabel that runs more slowly than the free DNA is directly proportional to the amount of transcription factor present.

Dictionary of molecular biology. 2004.

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